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Image Search Results
Journal: Journal of immunology (Baltimore, Md. : 1950)
Article Title: TCR Retrogenic Mice as a Model to Map Self-Tolerance Mechanisms to the Cancer Mucosa Antigen Guanylyl Cyclase C (GUCY2C)
doi: 10.4049/jimmunol.1801206
Figure Lengend Snippet: A–C) Bone marrow was isolated from donor Rag1−/− mice and HSCs were purified by negative selection of lineage-positive cells. Purified HSCs were left untransduced or transduced 1–3 times with DO11 or GUCY2C TCRs. A) HSC expansion ex vivo. B) Transduced HSCs remained lineage negative (mature lineage markers B220, Ter119, CD4, and Gr-1 shown here) following retroviral transduction and expansion, comparable to HSCs stained directly ex vivo. C) HSC transduction efficiency following 1, 2, or 3 applications of TCR retrovirus (1x, 2x or 3x). Values indicate percentage of GFP+ cells. Data are representative of 3 experiments.
Article Snippet: Briefly, Rag1 − /− mice were injected i.p. with 150 mg/kg of 5-fluorouracil (Millipore Sigma); bone marrow was harvested 72 h later and murine HSCs were purified using
Techniques: Isolation, Purification, Selection, Ex Vivo, Transduction, Staining